Optimal Conditions for Translation by Thylakoid-Bound Polysomes from Pea Chloroplasts

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Optimal conditions for translation by thylakoid-bound polysomes from pea chloroplasts.

Polysomes bound to washed thylakoids from pea Pisum sativum cv Progress No. 9 chloroplasts are capable of protein synthesis when supplemented with amino acids, ATP and a regenerating system, GTP, and soluble factors required for translation. The extent of protein synthesis in previous reports, however, was quite low when compared to in organello translation. By systematic testing of parameters ...

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ژورنال

عنوان ژورنال: Plant Physiology

سال: 1984

ISSN: 0032-0889,1532-2548

DOI: 10.1104/pp.75.3.832